We welcome you to join this Webinar series, where you will experience a variety of live mass spec-based presentations from top-ranked scientists on the wide-ranging  applications  of  stable isotopes. These include proteomics, metabolomics, clinical applications and more! 

 

 

October 28, 2021 11:00 AM - 12:00 PM EST

Combining Multidimensional Measurements with Standards and Isotopologue Workflows to Detect, Identify and Validate Molecules in Omic Studies

Erin Baker, PhD | Associate Professor
North Carolina State University

 Abstract: While the selectivity and specificity of LC-MS/MS methods have become increasingly powerful for feature annotation in untargeted analyses, previous studies have shown that in metabolomics analyses only a small percentage of detected features are actually metabolites occurring from the system and many features result from in-source fragments, multimers, or other artifacts of the MS experiment. This presentation will illustrate two different small molecule analysis pipelines utilized by our group to investigate features from untargeted studies. First, I will demonstrate how combining liquid chromatography, ion mobility spectrometry and tandem mass spectrometry (LC-IMS-MS/MS) separations with isotopologue workflows, enables the detection, identification and validation of features associated with metabolism. Next, I will showcase how we utilize standards and the LC-IMS-MS/MS measurements to create multidimensional libraries providing additional confidence to our feature annotations. I will then apply our bile acid library containing >200 molecular entries to examine how novel bile acid conjugates change due to specific system perturbations.

During this webinar you will learn about:

  • The power of multidimensional liquid chromatography, ion mobility spectrometry and tandem mass spectrometry platforms for untargeted analyses
  • How isotopologue workflows help pinpoint features related to metabolism
  • The utility of multidimensional libraries for narrowing down potential feature identifications

 


November 17, 2021 11:00 AM - 12:00 PM EST

Metabolomics for Molecular Diagnostics in Urinary Cancers

Tim Garrett, PhD | Associate Professor
University of Florida

Abstract: An estimated 248,530 American men will be diagnosed with prostate cancer in 2021 and roughly, 34,000 will die. As with many malignancies, the impact of a urologic cancer on a patient’s life expectancy and quality of life is largely based upon cancer stage. Early stage prostate cancer (stage I and II) is associated with excellent cancer specific outcomes and tremendous emphasis is placed on limiting treatment related morbidity and preserving quality of life. However, several challenges must be addressed to avoid overtreatment and unnecessary testing. A reliable assessment of early stage prostate cancer is vital for identifying suitable treatment options, as is a diagnostic test that has biomarkers specific to prostate. One of the failures of biomarker translation to clinical practice is utilizing specimens that do not represent the broad clinical phenotype present in a patient population for biomarker identification and testing. Thus, a true biomarker validation study should include patients with a wide spectrum of urologic conditions so the true accuracy and precision of the biomarker can be assessed. Metabolites represent the closest aspect to phenotype because they are utilized in healthy and disease processes. In fact, many current clinical tests rely on metabolites for health diagnostics (i.e. comprehensive metabolic panel). Our preliminary metabolomics show that urine biomarkers can identify prostate cancer and calibrate Gleason score (severity) while also differentiating prostate cancer from prostatitis, BPH, bladder cancer, and kidney cancer.

During this webinar you will learn about:

  • Metabolomic profiling of urine can identify prostate cancer patients
  • Single metabolites can calibration disease severity
  • A diverse clinical population is necessary for biomarker identification

 


December 1, 2021 11:00 AM - 12:00 PM EST

Deep Proteome Sequence Analysis, Increasing Isobaric Tag Reporter Ion Signal Using IR Photons, and Coupling MS to Electron Microscopy

Josh Coon, PhD | Professor of Chemistry and Biomolecular Chemistry at University of Wisconsin-Madison

Abstract: In this seminar I will discuss three topics relevant to our latest efforts to develop mass spectrometric technologies for proteome analysis. First, I will present a multi-protease strategy that provides the deepest coverage of the human proteome to date with detection of over 17,000 gene products. By use of various enzymes and MS/MS dissociation technologies we obtain extremely high sequence coverage allowing for a global mapping of alternative splicing on the proteome level. Second, I will present on the use of an IR laser, coupled with ion parking in a quadrupole linear ion trap, to double the production of TMT reporter ions for quantitative proteomic experiments. This approach boosts the number of quantifiable peptides in a global experiment and overall improves the quantitative accuracy and precision. Finally, I will describe new approaches to soft landing intact protein complexes onto transmission electron microscope grids for structural analysis. This approach offers a direct path to connect the nascent field of native MS to cryoEM.

During this webinar you will learn about:

  • Multi-protease strategy to gain deep gene coverage of the human proteome using mass spectrometry
  • Use of an IR laser and ion parking to double production of TMT reporter ions for quantitative proteomics
  • New approach to soft landing intact protein complexes onto transmission electron microscope grids for structural analysis

 

Isotope Day is a virtual event hosted on ZOOM. Space is limited. 

Biographies

Erin Baker, PhD | Associate Professor North Carolina State University

Erin S. Baker is an Associate Professor at North Carolina State University in Raleigh, NC. To date, she has published over 140 peer-reviewed papers utilizing ion mobility spectrometry in conjunction with mass spectrometry (IMS-MS) to study both environmental and biological systems. Erin has served on the ASMS Board of Directors as the Member at Large for Education and is currently serving as the Vice President of Education for the International Lipidomics Society, Events Committee Chair for Females in Mass Spectrometry (FeMS) and as an Associate Director in the NCSU Comparative Medicine Institute. She is also an Associate Editor for the Journal of the American Society for Mass Spectrometry and on the Advisory Board for Journal of Proteome Research and Analytical and Bioanalytical Chemistry. She has received seven US patents, two R&D 100 Awards, been named to the Analytical Scientist 2019 Top 100 Power List, aided in the commercialization of the Agilent 6560 IMS-QTOF MS, and was a recipient of the 2016 ACS Rising Star Award for Top Midcareer Women Chemists. Currently, her research group utilizes advanced separations, multi-omic analyses and novel software capabilities to examine connections between human health and the environment.

Tim Garrett, PhD | Associate Professor University of Florida

LoreDr. Garrett is Chief of Experimental Pathology in the Department of Pathology, Immunology and Laboratory Medicine at the College of Medicine at The University of Florida. In addition to his role as chief, Dr. Garrett has served as PI or Co-PI on grants and contracts totaling over $39M of funding from institutions such as the NIH, JDRF and the ADA. As an associate professor, Dr. Garrett has supervised the research of 18 graduate students and 3 post-doctoral students during the past 15 years, and received the term professorship in 2018, an exemplary teacher award in 2019, and the IFAS high impact publication in 2016. Research in the group has led to over 100 peer-reviewed publications with an h-index of 27 and an i10-index of 72. His publications have resulted in over 3000 citations and over 100 lectures in 6 countries relating to his research findings. Recent discoveries of note include the development a rapid spray system for imaging mass spectrometry (Patented), the development of open source processing tools for lipid identification (LipidMatch), an improved technique for identification of low abundant metabolites (Iterative Exclusion-Omics), and new work that will translation prostate cancer biomarkers to clinical diagnostics. 
Dr. Garrett received his undergraduate degree from the University of Georgia in Chemistry and his PHD in Chemistry from the University of Florida. As an undergraduate student, he received the Howard Hughes Fellowship in 1998 with Dr. Jonathan Amster.  As a graduate student, he worked on the development and design the first imaging mass spectrometry ion trap instrument through a partnership with Thermo (now Thermo Fischer Scientific) and studied the disposition and characterization of phospholipids in brain tissue. He received the Crow-Stasch Award for graduate research in 2006 and the International Journal of Mass Spectrometry award for publication in 2007.  He joined the faculty at the University of Florida in 2006, where he has developed a research program in metabolomics, lipidomics, and small molecule quantitation for clinical and biological research using mass spectrometry approaches. Dr. Garrett is also the Chief Scientific Officer of Juno Metabolomics, a private company specializing in rapid, reliability, and cloud-based analytics in metabolomics and diagnostics.

Josh Coon, PhD | Professor of Chemistry and Biomolecular Chemistry at University of Wisconsin-Madison

Hailing from a small town in central Michigan, Coon escaped from the cold to earn a Ph.D. at the University of Florida. He went on to conduct his postdoctoral studies with Don Hunt at the University of Virginia. During that time he, along with Hunt and John Syka, co-invented electron transfer dissociation. In 2005 he joined the faculty at Wisconsin to start his own program. Coon’s research group aims to advance mass spectrometer technology to make proteome analysis faster and more accessible. Coon’s research in these areas has been recognized by several awards including the Biemann Medal from the American Society for Mass Spectrometry and the Ken Standing Award from the University of Manitoba.  He is the Director of the NIGMS funded National Center for Quantitative Biology of Complex Systems.